Mechanism Of Reduction Of An Aminyl Radical Intermediate In The Radical Sam Gtp 3 ',8-Cyclase Moaa

The diversity of the reactions catalyzed by radical S-adenosyl-L-methionine (SAM) enzymes is achieved at least in part through the variety of mechanisms to quench their radical intermediates. In the SPASM-twitch family, the largest family of radical SAM enzymes, the radical quenching step is thought to involve an electron transfer to or from an auxiliary 4Fe-4S cluster in or adjacent to the active site. However, experimental demonstration of such functions remains limited. As a representative member of this family, MoaA has one radical SAM cluster ([4Fe-4S](RS)) and one auxiliary cluster ([4Fe-4S](AUX)), and catalyzes a unique 3',8-cyclization of GTP into 3',8-cyclo-7,8-dihydro-GTP (3',8-cH(2)GTP) in the molybdenum cofactor (Moco) biosynthesis. Here, we report a mechanistic investigation of the radical quenching step in MoaA, a chemically challenging reduction of 3',8-cyclo-GTP-N7 aminyl radical. We first determined the reduction potentials of [4Fe-4S](RS) and [4Fe-4S](AUX) as -510 mV and -455 mV, respectively, using a combination of protein film voltammogram (PFV) and electron paramagnetic resonance (EPR) spectroscopy. Subsequent Qband EPR characterization of 5'-deoxyadenosine C4' radical (5'-dA-C4'center dot) trapped in the active site revealed isotropic exchange interaction (similar to 260 MHz) between 5'-dA-C4'center dot and [4Fe-4S](AUX)(1+), suggesting that [4Fe-4S](AUX) is in the reduced (1+) state during the catalysis. Together with density functional theory (DFT) calculation, we propose that the aminyl radical reduction proceeds through a proton-coupled electron transfer (PCET), where [4Fe-4S](AUX) serves as an electron donor and R17 residue acts as a proton donor. These results provide detailed mechanistic insights into the radical quenching step of radical SAM enzyme catalysis.