Rapid unfolding of pig pancreas α-amylase: Kinetics, activity and structure evolution
Food Chemistry(2022)
Abstract
•Kinetics of rapid unfolding of α-amylase was disclosed on the microsecond scale.•Detailed structural changes of α-amylase during the unfolding was described.•Unfolding of α-amylase from pig pancreas by guanidine hydrochloride was studied.•Contribution of H-bond structure of water to protein unfolding was disclosed.•One intermediate existed in the unfolding of pig pancreas α-amylase.
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Key words
Pig pancreas α-amylase,Unfolding,Guanidine hydrochloride,Stopped-flow fluorescence
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