Sinanaerobacter chloroacetimidivorans gen. nov., sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge

An obligate anaerobic bacterial strain (BAD-6 T ) capable of degrading acetochlor and butachlor was isolated from an anaerobic acetochlor-degrading reactor. Cells were Gram-stain positive, straight to gently curved rods with flagella. The major fermentation products in peptone-yeast broth were acetate and butyrate. The optimum temperature and pH for growth was 30 °C and 7.2–7.5, respectively. The major cellular fatty acids (> 10%) were C 14:0 FAME, C 16:0 FAME and cyc-9,10-C 19:0 DMA. Genome sequencing revealed a genome size of 4.80 Mb, a G + C content of 43.6 mol% and 4741 protein-coding genes. The most closely related described species on the basis of 16S rRNA gene sequences was Anaerovorax odorimutans NorPut T in the order Clostridiales of the class Clostridia with sequence similarity of 94.9%. The nucleotide identity (ANI) value and digital DNA–DNA hybridization (dDDH) between the genomes of strain BAD-6 T and Ana. odorimutans NorPut T were 70.9% and 15.9%, respectively. Based on the distinct differences in phylogenetic and phenotypic characteristics between strain BAD-6 T and related species, Sinanaerobacter chloroacetimidivorans gen. nov., sp. nov. is proposed to accommodate the strain. Strain BAD-6 T is the type strain (= CCTCC AB 2021092 T = KCTC 25290 T ).