Ustekinumab Therapy Changes The Transcriptional Activity Pattern Of Tgf-Beta 1-3 Genes

Introduction: One of the examples of genes whose expression can be altered by the action of ustekinumab is TGF-beta. It is a pleiotropic cytokine whose activity affects psoriatic changes and the state of homeostasis of the whole organism.Aim: To evaluate the effect of ustekinumab on the transcriptional activity of TGF-beta family genes in patients with psoriatic arthritis and to check whether the results obtained can be helpful in monitoring the progress of treatment.Material and methods: From total PBMCs obtained from peripheral blood of 14 patients with psoriatic arthritis, total RNA was isolated. The expression level of the TGF-beta 1, TGF-beta 2 and TGF-beta 3 genes was determined by RT-qPCR in real time.Results: In all the analysed samples, the presence of mRNA of three TGF-beta isoforms was quantitated in each week of therapy. TGF-beta 3 and the smallest TGF-beta 2 showed the highest expression. Statistically significant correlations were observed in the amount of TGF-beta 1 and TGF-beta 3/mu g mRNA RNA, TGF-beta 2 and TGF-beta 2/mu g RNA and TGF-beta 3 and TGF-beta 3/mu g RNA.Conclusions: Ustekinumab influences the transcriptional activity of TGF-beta genes, and the changes caused have a bearing on the patient's health.