Hydrogen Attenuates Myocardial Injury In Rats By Regulating Oxidative Stress And Nlrp3 Inflammasome Mediated Pyroptosis

Purpose: Hydrogen (H-2) is an antioxidant with anti-inflammatory and apoptosis functions.This study aimed to estimate the effects of H-2 on acute myocardial infarction (AMI) in rats and its association with the inhibition of oxidative stress and cardiomyocyte pyroptosis.Methods: Sixty-four rats were randomly divided into three groups (Sham, AMI, and H-2). The left anterior descending coronary artery (LAD) of rats in the AMI and H-2 groups was ligated, while rats in the Sham group were threaded without ligation. In addition, 2% H-2 was administered by inhalation for 24 h after ligation in the H-2 group. Transthoracic echocardiography was performed after H-2 inhalation, followed by collection of the serum and cardiac tissue of all rats.Results: H-2 inhalation ameliorated the cardiac dysfunction, infarct size and inflammatory cell infiltration caused by AMI. Meanwhile, H-2 inhalation reduced the concentration of serum Troponin I (TnI), brain natriuretic peptide (BNP), reactive oxygen species (ROS), cardiac malondialdehyde (MDA), and 8-OHdG. In addition, H-2 inhalation inhibited cardiac inflammation and pyroptosis relative proteins expression.Conclusion: H-2 effectively promoted heart functions in AMI rats by regulating oxidative stress and pyroptosis.