Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing
Molecular genetics and metabolism（2021）
Abstract
CRISPR/Cas9-based technology has revolutionized biomedical research by providing a high-fidelity gene-editing method, foreshadowing a significant impact on the therapeutics of many human genetic disorders previously considered untreatable. However, off-target events represent a critical hurdle before genome editing can be fully established in clinical practice. This mini-review recapitulates some recent advances for detecting and overcoming off-target effects mediated by the CRISPR/Cas9 system that could increase the likelihood of clinical success of the CRISPR-based approaches. (c) 2021 Elsevier Inc. All rights reserved.
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Key words
CRISPR,Cas9,Genome-editing,Off-target effects,Base editors,Prime editing,Anti-CRISPR proteins
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