Application of sea elastase myroilysin in preparation of acellular dermal matrix

The invention relates to application of sea elastase myroilysin in the preparation of an acellular dermal matrix, and the preparation of the acellular dermal matrix comprises the following steps: (1) performing fermentation culture of an activated myroides profundi D25 strain, centrifugating, taking a supernatant, adding ammonium sulfate, collecting a precipitation, centrifugating after redissolving and dialysis, separating and purifying by ion exchange chromatography to obtain the elastase myroilysin; (2) dissolving the elastase myroilysin in a Tris-HCl buffer solution to prepare an enzyme solution; (3) dipping fatty layer and epidermis removed pigskin in the enzyme solution for processing, and taking out for washing to prepare the pigskin acellular dermal matrix. The sea-derived elastase myroilysin has high acellular and elastase removal efficiency, is fast in speed and small in use amount; and the prepared acellular dermal matrix is large in aperture and high in porosity, facilitates cell entry and proliferation in tissue engineering, and has wide uses in the tissue engineering.