Specific detection of memory T-cells in COVID-19 patients using standardized whole-blood Interferon gammarelease assay

William Mouton,Christelle Compagnon,Kahina Saker,Soizic Daniel,Sophia Djebali,Xavier Lacoux,Bruno Pozzetto,Guy Oriol,Daphné Laubreton,Margaux Prieux,Jean-Baptiste Fassier,Nicolas Guibert,Amélie Massardier-Pilonchéry,Dulce Alfaiate,Franck Berthier,Thierry Walzer,Jacqueline Marvel,Karen Brengel-Pesce,Sophie Trouiller-Assant

EUROPEAN JOURNAL OF IMMUNOLOGY（2021）

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摘要

Antigen-specific T-cells are essential for protective immunity against SARS-CoV-2. We set up a semi-automated whole-blood Interferon-gamma release assay (WB IGRA) to monitor the T-cell response after stimulation with SARS-CoV-2 peptide pools. We report that the WB IGRA is complementary to serological assays to assess SARS-CoV-2 immunity.

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关键词

immune memory, interferon gamma release assay, SARS-CoV-2, T-cells response

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