Drosophila Suzukii Susceptibility To The Oral Administration Of Bacillus Thuringiensis, Xenorhabdus Nematophila And Its Secondary Metabolites

Simple Summary In recent decades, climate change and the international fruit trade have favored the movement of allochthonous species such as harmful insects into new geographic areas. The settlement of phytophagous insects and vectors in new areas, where potential predators are often lacking, has increased the use of chemical insecticides for their control. The intensive use of these substances represents a serious problem for ecosystems and human health; a possible alternative to chemical control is biological control, i.e., the use of biological insecticides that are compatible with the environment. The aim of our work was to further improve biological control methods for the management of the dipteran Spotted Wing Drosophila, an insect recently introduced in America and Europe, which can damage thin-skinned fruit crops. The methodologies applied are based on the combined use of different entomopathogens, i.e., bacteria, fungi, nematodes, etc., harmful for insects, with the purpose of increasing their effectiveness. The results obtained show that the combined use of two entomopathogenic bacteria increases both the lethality and rapidity of action. From an application viewpoint, studies like this are essential to identify new methods and bioinsecticides and, once transferred to the field, can be crucial to eliminate or, at least, reduce the use of chemicals. Drosophila suzukii, Spotted Wing Drosophila (SWD), is a serious economic issue for thin-skinned fruit farmers. The invasion of this dipteran is mainly counteracted by chemical control methods; however, it would be desirable to replace them with biological control. All assays were performed with Bacillus thuringiensis (Bt), Xenorhabdus nematophila (Xn), and Xn secretions, administered orally in single or combination, then larval lethality was assessed at different times. Gut damage caused by Bt and the influence on Xn into the hemocoelic cavity was also evaluated. In addition, the hemolymph cell population was analyzed after treatments. The data obtained show that the combined use of Bt plus Xn secretions on larvae, compared to single administration of bacteria, significantly improved the efficacy and reduced the time of treatments. The results confirm the destructive action of Bt on the gut of SWD larvae, and that Bt-induced alteration promotes the passage of Xn to the hemocoel cavity. Furthermore, hemocytes decrease after bioinsecticides treatments. Our study demonstrates that combining bioinsecticides can improve the efficacy of biocontrol and such combinations should be tested in greenhouse and in field in the near future.