New coumarin glycosides from endemic Prangos heyniae H. Duman & M.F. Watson.

Two new coumarin glycosides, named 7-methoxy isoarnottinin 4'--ᴅ-glucopyranoside and 7-methoxy isoarnottinin 4'-rutinoside ( and ) along with six known compounds () were isolated from the roots of an endemic plant of Turkey. 1-methylethyl 6--D-apio--ᴅ-furanosyl--ᴅ-glucopyranoside () and cnidioside A () have been obtained from the genus for the first time. Structures of isolated compounds were established using spectroscopic methods (1 D and 2 D NMR, HR-MS, UV and IR). Moreover, all extracts and isolated compounds were evaluated for their cytotoxic activity against NIH/3T3, HK-2, A-549, MCF-7, PC-3 and SH-SY5Y cell lines by WST-1 method. One of the new coumarin glycosides, 7-methoxy isoarnottinin 4'--ᴅ-glucopyranoside () exhibited selective cytotoxic activity against SH-SY5Y cells with IC value of 31.41 ± 1.04 μM.