Pulsed Ultrasound Prevents Lipopolysaccharide-Induced Muscle Atrophy Through Inhibiting P38 Mapk Phosphorylation In C2c12 Myotubes
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(2021)
摘要
Objective: Inflammation contributes to skeletal muscle atrophy via protein degradation induced by p38 mitogen-activated protein kinase (MAPK) phosphorylation. Meanwhile, pulsed ultrasound irradiation provides the mechanical stimulation to the target tissue, and has been reported to show anti-inflammatory effects. This study investigated the preventive effects of pulsed ultrasound irradiation on muscle atrophy induced by lipopolysaccharide (LPS) in C2C12 myotubes.Methods: C2C12 myotubes were used in this research. The pulsed ultrasound (a frequency of 3 MHz, duty cycle of 20%, intensity of 0.5 W/cm(2)) was irradiated to myotube before LPS administration.Results: The LPS increased phosphorylation of p38 MAPK and decreased the myofibril and myosin heavy chain protein (P < 0.05), followed by atrophy in C2C12 myotubes. The pulsed ultrasound irradiation attenuated p38 MAPK phosphorylation and myotube atrophy induced by LPS (P < 0.05).Conclusions: Pulsed ultrasound irradiation has the preventive effects on inflammation-induced muscle atrophy through inhibiting phosphorylation of p38 MAPK. (C) 2021 Elsevier Inc. All rights reserved.
更多查看译文
关键词
Pulsed ultrasound irradiation, Muscle atrophy, Lipopolysaccharide, p38 MAPK, C2C12 myotubes
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要