Overexpression of the Long Noncoding RNA HIF2PUT Inhibits Non-Small Cell Lung Cancer Cell Proliferation and Invasion Through HIF-2a Pathway.

The role and molecular mechanism of long-chain noncoding RNAs (lncRNAs) in lung cancer remain to be elucidated. The aim of this study was to investigate the association between a long coding RNA hypoxia-inducible factor-2α (HIF-2α) promoter upstream transcript () and clinical characteristics of non-small cell lung cancer (NSCLC) and its regulatory role in NSCLC. The correlation between expression and pathological features of NSCLC was analyzed in NSCLC patient samples. Real-time polymerase chain reaction and Western blot were used to detect genes' mRNA and protein expression, respectively. Cell proliferation assay, invasion, and transwell assay were performed to determine the effects of on NSCLC cells. lncRNA was downregulated in NSCLC tissues and cell lines. The authors also indicated that mainly expressed in cytoplasm and overexpression of attenuates cell proliferation and invasion in NSCLC cells. Moreover, low expression of was significantly related to TNM stage ( = 0.045) and histological type ( = 0.025). Furthermore, they found that plays role in cell proliferation and invasion in NSCLC through regulating HIF-2a. Based on the study, the inhibitory role of on NSCLC proliferation, invasion could be blocked by HIF-2a silencing. In summary, this study suggests that and HIF-2a may play an important role in the regulation of NSCLC progression, which provides new insights for clinical treatment.