Fed-batch production assessment of a tetravalent bispecific antibody: A case study on piggyBac stably transfected HEK293 cells

Antonino Napoleone,Ida Laurén, Triinu Linkgreim,Leif Dahllund,Helena Persson,Oskar Andersson,Anders Olsson,Greta Hultqvist, Peter Frank, Martin Hall, Annika Morrison, Andreas Andersson,Martin Lord,Sara Mangsbo

New Biotechnology（2021）

引用 11|浏览8

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摘要

•PiggyBac expression of an intrinsically complex tetravalent bispecific antibody.•Affordable large-scale protein production process development.•Fed-batch protocol achieving 90 % viability over 12 days post.•Novel high-speed protein A fiber technology reduced capture residence time to 7.5 s.

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关键词

mAb,CMO,CHO cells,PTMs,HEK cells,ITR,scFv,GMP,MCS,CMV,GFP,ELISA,HRP,TMB,SPR,LDH,CIP,CV,SEC,DBC

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