Design and Adaptation of Robust Feeding Profiles for Recombinant E.coli Fed-batch Cultivation Processes*

In this paper, the experimental data-based procedure for design and adaptation of robust feeding profiles for recombinant E.coli fed-batch cultivation processes has been proposed. In the first step of the procedure, a maximum glucose consumption rate in biomass growth and target protein production phases must be determined using data from an unlimited growth cultivation. Afterwards, various scenar...