Analysis of the Secondary Metabolism in Magnaporthe oryzae.

Magnaporthe oryzae produces a number of secondary metabolites, some of which are thought to be responsible for the virulence of this fungus toward rice. Due to the importance of understanding plant-pathogen interactions, several of these metabolites have been investigated chemically and biosynthetically. This chapter provides an overview of the secondary metabolites isolated from M. oryzae and describes a general method for metabolite extraction, followed by an analysis using high-performance liquid chromatography (HPLC) combined with mass spectrometry (LCMS).