Characterization of Total and Unprotonated (Free) Nicotine Content of Nicotine Pouch Products

Introduction: Nicotine pouch products, oral smokeless products that contain nicotine but no tobacco leaf material, have recently entered the US marketplace. Available data indicate sales of these products in the United States have increased since 2018; however, the extent of use among US youth and adults is uncertain. Methods: To assay the chemistry of these emerging tobacco products, we analyzed 37 nicotine pouch brands from six total manufacturers. Almost all of the products had flavor descriptors (36 of 37), such as mint, licorice, coffee, cinnamon, and fruit.The amount of free nicotine, the form most easily absorbed, was calculated for each product using total nicotine, product pH, the appropriate pK(a), and the Henderson-Hasselbalch equation. Results: Nicotine pouch products varied in pouch content mass, moisture content (1.12%-47.2%), alkalinity (pH 6.86-10.1), and % free nicotine (7.7%-99.2%). Total nicotine content ranged from 1.29 to 6.11 mg/pouch, whereas free nicotine ranged from 0.166 to 6.07 mg/pouch. These findings indicate that nicotine and pH levels found in some of these nicotine pouches are similar to conventional tobacco products, such as moist snuff and snus, and that most of these pouch products are flavored. Conclusions: Although these products likely lack many tobacco-related chemicals, each product analyzed contained nicotine, which is both addictive and can harm human health. Given that nicotine pouches may appeal to a spectrum of users, from novice to experienced users, it is important to include these emerging tobacco products in tobacco control research, policy, and practice.