Using Droplet Digital Pcr To Detect Plant Dna In Tissues Of Zebrafish (Danio Rerio) Fed Genetically Modified Maize
AQUACULTURE RESEARCH（2021）
Abstract
The global commercialization of genetically modified (GM) crops has increased the likelihood of feed-derived transgenic DNA uptake and raised concerns regarding the fate of such DNA fragments in animals consuming a GM diet. The present study applied a novel droplet digital polymerase chain reaction (ddPCR) method to quantitatively detect the concentrations of feed-derived transgenic (Cry1Ie) and endogenous (rubisco) plant DNA in the livers, intestinal tracts and muscles of zebrafish (Danio rerio) fed GM maize for uantifying feed-derived DNA in animal tissues as a more sensitive and reliable alternative to qPCR.
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Key words
Cry1Ie, droplet digital polymerase chain reaction (ddPCR), genetically modified maize, rubisco, the limit of quantification (LOQ), zebrafish
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