Ammonium Ions Induce Cellulase Synthesis in Trichoderma koningii
CURRENT MICROBIOLOGY(2021)
摘要
Cellulase plays an important role in addressing the issue of the energy crisis. However, the yield and degradation efficiency of cellulase remain a major challenge. In the present study, we aimed to verify whether ammonium ion (NH 4 + ) could induce cellulase synthesis from T. koningii AS3.2774 and to explore new functional genes related to the cellulase production. Our results indicated that NH 4 + induces cellulase production in a way different from nitrogen sources. NH 4 + -mediated mycelia displayed a significant increase in transport vesicles. Under NH 4 + mediation, CBHI, CBHII, glycoside hydrolase family 5 proteins, Hap2/3/5 complexes, “ribosome biogenesis”, and “heme binding” were significantly up-regulated, and differentially expressed genes (DEGs) were mainly involved in “Metabolism”. Collectively, our findings illustrated that NH 4 + induced the cellulase production at morphological and gene expression levels, which might be related to the Hap2/3/5 complex, ribosomes, and genes involved in various amino acid metabolism, pyruvate metabolism, and glycolysis/gluconeogenesis. Taken together, our results provided valuable insights into the regulatory network of cellulase gene expression in filamentous fungi.
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关键词
cellulase,ammonium
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