Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines

During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcrip-tion factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRa. We detail procedures for HeLa cell synchronization followed by immunofluorescence, in situ proximity ligation assay, and centrosome isolation. This protocol can be used to identify other transcription factors associated with the centrosome or other subcellular structures during mitotic progression. For complete details on the use and execution of this protocol, please refer to Xie et al. (2020)