Microbial Sulfite Oxidation Coupled To Nitrate Reduction In Makeup Water For Oil Production

Bisulfite is used as an oxygen scavenger in waters used for oil production to prevent oxygen-mediated pipeline corrosion. Analysis of nitrate-containing water injected with ammonium bisulfite indicated increased concentrations of ammonium, sulfate and nitrite. To understand the microbial process causing these changes, water samples were used in enrichments with bisulfite and nitrate. Oxidation of bisulfite, reduction of nitrate, change in microbial community composition and corrosivity of bisulfite were determined. The results indicated that the microbial community was dominated by Sulfuricurvum, a sulfite-oxidizing nitrate-reducing bacterium (StONRB). Plating of the enriched StONRB culture yielded the bacterial isolate Sulfuricurvum sp. TK005, which coupled bisulfite oxidation with nitrate reduction to form sulfate and nitrite. Bisulfite also induced chemical corrosion of carbon steel at a rate of 0.28 +/- 0.18 mm yr(-1). Bisulfite and the generated sulfate could serve as electron acceptors for sulfate-reducing microorganisms (SRM), which reduce sulfate and bisulfite to sulfide. Nitrate is frequently injected to injection waters to contain the activity of SRM in oil reservoirs. This study suggests an alternative bisulfite injection procedure: Injection of nitrate after the chemical reaction of bisulfite with oxygen is completed. This could maintain the oxygen scavenger function of bisulfite and SRM inhibitory activity of nitrate.