Mycobacterium Tuberculosis Protein Ppe2 Binds To Dna Region Containing Promoter Activity
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(2021)
Abstract
ppe2 gene is predicted to be present in operon with non pe/ppe genes, cobq1 and cobu as ppe2-cobq1-cobu. Thus, we were interested to investigate the role of ppe2 in operonic organization. We performed microscale thermophoresis (MST) experiment which revealed that PPE2 protein could bind to upstream DNA segments of ppe2-cobq1-cobu operon. Upstream region of ppe2 had shown promoter activity in beta-gal assay. In this study, for the first time, a physical interaction between PPE2 protein and DNA fragment was reported, suggesting that PPE2 protein plays a role in the regulation of the putative ppe2-cobq1-cobu operon, via unknown mechanism. (C) 2021 Elsevier Inc. All rights reserved.
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Key words
PPE2 protein, Mycobacterium tuberculosis, Operon, Promoter, Microscale thermophoresis (MST), DNA-Protein interaction
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