Crispr/Cas9-Mediated Genome Editing For Wheat Grain Quality Improvement

Common wheat supply vast amounts of dietary carbohydrate and protein for over 60% of world population. With continued economic development, demand for high yielding varieties with premium grain quality is increasing. Grain quality is a multigenic trait that is simultaneously affected by many factors, and it's more complicated in hexaploid wheat. CRISPR/Cas9-based genome editing provides great opportunities to create more allelic variations in a much faster and precise manner (Li et al., 2020a,b; Sanchez-Leon et al., 2018; Zhang et al., 2020a,b). In this study, four grain-quality related genes viz. pinb, waxy, ppo and psy, which involved in wheat grain hardness, starch quality, and dough color, respectively, were targeted for genome editing.