Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine.

Methods in molecular biology (Clifton, N.J.)（2021）

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摘要

Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy.

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关键词

Fluorescence imaging,MitoSOX Red®,Mitochondrial membrane potential

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