Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat- Triticum boeoticum introgression line

Key message A novel recessive gene YrZ15-1370 derived from Triticum boeoticum confers adult–plant resistance to wheat stripe rust. Abstract Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy. Triticum boeoticum Boiss ( T. monococcum L. ssp. aegilopoides , 2 n = 2 x = 14, A b A b ) accession G52 confers a high level of adult–plant resistance against a mixture of the Chinese prevalent Pst races. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat- T. boeoticum introgression line Z15-1370 (F 5 generation) with 42 chromosomes was selected cytologically and by testing with Pst races. F 1 , F 2 , and F 2:3 generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, tentatively designated YrZ15-1370 . Using the bulked segregant RNA-Seq (BSR-Seq) analysis, YrZ15-1370 was mapped to chromosome 6AL and flanked by markers KASP1370-3 and KASP-1370-5 within a 4.3 cM genetic interval corresponding to 1.8 Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated. YrZ15-1370 differed from previously Yr genes identified on chromosome 6A based on its position and/or origin. The YrZ15-1370 would be a valuable resource for wheat resistance improvement and the flanking markers developed here could be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene.