A Fast PCR Test for the Simultaneous Identification of Species and Gender in Horses, Donkeys, Mules and Hinnies

•This PCR test to identify the species and gender in horses, donkeys, mules and hinnies only needs three pairs of primers and is just based on simple PCR.•This is the first time utilizing the mutation on equine autosome gene to identify horse, donkey and their hybrids DNA.•Our simple PCR method could be a cheap, fast and effective strategy to identify species and gender within horse, donkey, mule and hinny.