Antioxidant And Immunomodulatory Activities In Vitro Of A Neutral Polysaccharide From Ginger (Zingiber Officinale)

Hou Mingda, Gao Jun, Liu Ziqi,Shan Yu,Liu Shuyong

STARCH-STARKE(2021)

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摘要
In this study, crude polysaccharides from ginger are obtained by ultrasonic-assisted enzymatic extraction. After deproteinization using Sevag method, dialysis, and isolation on a DEAE52-cellulose column and a Sephadex G-100 column, a neutral heteropolysaccharide purified ginger polysaccharide (pGP) with the molecular weight of 62 500 Da is obtained. Four sugar residues are confirmed in pGP, including -> 3)-alpha-L-Araf-(1 ->, -> 2)-alpha-L-Rhap, -> 3)-beta-D-Galp-(1 ->, -> 6)-alpha-D-Glcp-(1 ->. Antioxidant activities analysis confirms that pGP possess good scavenging effects on DPPH, 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, and hydroxyl radicals. In addition, pGP significantly stimulates the phagocytic activity of RAW264.7 cells and has strong protection effect against H2O2-induced damage. These results show that pGP can be used as a potential antioxidant agent in medicine or functional food.
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关键词
antioxidant activity, ginger, immunomodulatory activity, polysaccharides, structures
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