Enhanced enantioseparation performance in cyclodextrin-electrokinetic chromatography using quinine modified polydopamine coated capillary column

•The use of immobilized quinine auxiliary modifier in CD-EKC is reported.•A novel chiral alkaloid-inducing one-pot coating strategy for CD-EKC was presented.•Greatly enhanced enantioseparation of eight enantiomers was achieved by the method.•The universal method can facilitate the evolution and applicability of CD-EKC.