Separation of human granulocytes and mononuclear cells from whole blood using percoll on a centrifugal microfluidic disc

•A novel of lab on a disc is designed for the separation of whole-blood components.•The applied strategy is based on the siphon crest height and capillary vales.•Using two DG’s allowed clean isolation of the cells without loss or contamination.•The harvested cells easily removed and counted without any troublesome steps.•The centrifugal disk provides an easy, cheap, and quick whole blood analysis.