Ex Situ Preservation In Medium-Term Culture Of The Threathened Taxon Dianthus Nardiformis Janka
ROMANIAN BIOTECHNOLOGICAL LETTERS(2021)
摘要
Our aim was to elaborate an efficient and reproducible protocol for medium-term culture of the threatened taxon Dianthus nardiformis. To reduce the growth, sucrose, mannitol, polyethylene glycol, Abscisic acid and Jasmonic acid were tested. For assessing the in vitro response, the growth and regeneration were registered after different time intervals.Mannitol is the most effective for medium-term preservation viable cultures which can be maintained unlimited time through transfer at every 3 months. In its presence, somatic embryogenesis was induced and in vitro growth in the minimal cultures was reduced between 9 and 12 times comparing to the control.Antioxidant enzymes assay revealed qualitative and quantitative differences among the experimental variants, and also between different concentrations of the same compound in correlation with the growth reduction and regeneration. PDX was the most suitable to detect the efficiency of different treatments to induce medium-term cultures.
更多查看译文
关键词
Medium-term, mannitol, somatic embryogenesis, antioxidant enzymes
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要