A Short Review, Effect Of Dimethyl-Beta-Cyclodextrin On The Interaction Between Helicobacter Pylori And Steroidal Compounds

The 2,6-di-O-methyl-beta-cyclodextrin (dM beta CD) is an amphiphilic annular compound consisting of seven dimethyl-glucose molecules. This compound is well known as a solubilizer of lipophilic compounds. Especially, dM beta CD extracts cholesterol from the plasma membrane of mammalian cells and releases the cholesterol to the aqueous solution. The experimental use of dM beta CD, therefore, serves to investigate the role of cholesterol in the mammalian cell membrane. It is, however, unclear as to how dM beta CD extracts cholesterol incorporated into the glycerophospholipid biomembrane. Meanwhile, dM beta CD acts as a beneficial compound for Helicobacter pylori and is used as the standard component for supporting the growth of this bacterium in the serum-free culture. However, the detailed mechanism of dM beta CD for supporting the growth of H. pylori is still to be clarified. H. pylori is a Gram-negative microaerophilic bacillus recognized as a pathogen concerned with gastrointestinal diseases in human. Previous studies by our group have successfully obtained the H. pylori strains culturable without dM beta CD and demonstrated the distinct effects of dM beta CD on the interaction between H. pylori and exogenous steroidal compounds. For instance, dM beta CD promotes and inhibits the absorption of cholesterol and several steroidal compounds respectively into the biomembranes of H. pylori. In this study we summarized behaviors of dM beta CD toward steroidal compounds relevant to H. pylori.