Improving of {\sl in vitro} plant regeneration frequency of {\sl Rosa canina} (Rosaceae) inermis
Acta Botanica Yunnanica(2006)
Abstract
An efficient regeneration system of Rosa canina Inermis was developed in this study. The results showed that the regeneration frequency of adventitious buds was 87% and the multiplication ratio was 3.0 when supplemented with 2.0 mg/L 6-BA and 0.3 mg/L 2,4 - D on Murashige and Skoog (MS) medium, while the regeneration ratio was 93% and the multiplication ratio was 5.0 when the MS medium contained 1.5 mg/L CPPU and 0.2 mg/L 2,4 - D. Moreover, the result also indicated that CPPU was more effective in the induction of adventitious buds. The optimal medium for the multiplication of shoots was MS + 40 g/L sucrose + 6.0 g/L agar + 3.5 mg/L AgNO3 + 1.5 mg/L CPPU + 0.1 mg/L 2,4 - D + 0.05 mg/ L GA3, in which the multiplication ratio was 5.5 and the adventitious bud induction frequency was 89%. The best medium for rooting and growing was 1/4MS + 20 g/L sucrose + 3.5 g/L agar + 0.3% activated carbon + 0.1 mg/L IBA + 0.1 mg/ LNAA. The rooting frequency was 91%.
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Key words
plant regeneration frequency,vitro}
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