Improving of {\sl in vitro} plant regeneration frequency of {\sl Rosa canina} （Rosaceae） inermis

An efficient regeneration system of Rosa canina Inermis was developed in this study. The results showed that the regeneration frequency of adventitious buds was 87% and the multiplication ratio was 3.0 when supplemented with 2.0 mg/L 6-BA and 0.3 mg/L 2,4 - D on Murashige and Skoog （MS） medium, while the regeneration ratio was 93% and the multiplication ratio was 5.0 when the MS medium contained 1.5 mg/L CPPU and 0.2 mg/L 2,4 - D. Moreover, the result also indicated that CPPU was more effective in the induction of adventitious buds. The optimal medium for the multiplication of shoots was MS ＋ 40 g/L sucrose ＋ 6.0 g/L agar ＋ 3.5 mg/L AgNO3 ＋ 1.5 mg/L CPPU ＋ 0.1 mg/L 2,4 - D ＋ 0.05 mg/ L GA3, in which the multiplication ratio was 5.5 and the adventitious bud induction frequency was 89%. The best medium for rooting and growing was 1/4MS ＋ 20 g/L sucrose ＋ 3.5 g/L agar ＋ 0.3% activated carbon ＋ 0.1 mg/L IBA ＋ 0.1 mg/ LNAA. The rooting frequency was 91%.