Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS

A-Type and B-type oligomeric proanthocyanidins were prepared from the 70% ethanol extract of peanut skin by purification on AB-8 macroporous adsorption resin and two fractions PSP-3 and PSP-5 were separated and identified by reversed phase high-performance liquid chromatography-tandem mass spectrometry(RP-HPLC-MS/MS). Peanut skin procyanidins(PSP) with a purity of over 98.0% and a recovery of 9.8% were obtained after the purification. PSP-3 was composed of 2 B-ty pe procyanidolic dimers and PSP-5 was composed of 4 A-type procyanidolic dimers.