Establishment of a multidrug resistance cell line from human laryngeal cancer cells and its biologic features

Objective:To establish a multidrug resistance cell line from human laryngeal cancer cells by VCR.Methods:Human laryngeal cancer cells(Hep-2) were exposed in stepwise escalating concentration of VCR until the resistant cell(Hep-2/v) line was developed.The IC50 and the resistance folds of multidrug resistance were detected with MTT assay.The differences of cell cycle distribution and Rhodamine accumulation between Hep-2 and Hep-2/v cells were studied through flow cytometry.The MDR1 gene were detected through real-time quantitative RT-PCR,and the corresponding protein was detected through western blot.Results:Hep-2/v cells was established,which had stable resistance to VCR and a resistance index of 45;Hep-2/v cells exhibited cross resistance to many other chemotherapeutic agents and its doubling time was prolonged;The number of cells in G0/G1 phase was increased while in S phase decreased(P0.05);Rhodamine accumulation in Hep-2 cells was much more than Hep-2/v cells(P0.01);The expression of MDR1 were increased than that of Hep-2 cells(P0.01).Conclusion:Hep-2/v cell line shows the typical multidrug resistance phenotype.It can be served as a model for the study of MDR.