Histopathological And Calreticulin Changes In The Liver And Gill Of Takifugu Fasciatus Demonstrate The Effects Of Copper Nanoparticle And Copper Sulphate Exposure

Copper nanoparticles (Cu-NPs) are water pollutants, which has significant toxic environmental effects. However, the effects of Cu-NPs on Takifugu fasciatus are still relatively unknown. Compared with the control (no add CuSO4 and Cu-NPs). This study aims to describe the pathological changes in the gill and liver of pufferfish exposed to CuSO4 (Cu2+ for short) and Cu-NPs dissolved at a concentration of either 20 or 100 mu g/L, in a semi-static waterborne exposure regime for 28 days. All Cu treatments resulted in organ injuries, and the pathologies observed following exposure to Cu-NPs were broadly of the same type as those observed following exposure to Cu2+. In the gill, these included lamellar fusion, clubbed tips, and mucocytes swollen, and in the liver included oedema, aggregation of blood cells, and necrosis. Furthermore, enzyme activity assays revealed that Ca2+ -ATP activity significantly increased under the influence of Cu, whilst Na+-K+-ATPase activity was significantly inhibited. In this study, the CRT in T. fasciatus (referred to as TfCRT) was identified and full-length cDNA sequences were characterized. Subsequently, RT-PCR and western blot analysis were carried out to assess the expressions of TfCRT in the gill and liver at both the mRNA and protein levels. Under both the Cu-NPs and Cu2+ treatments, both the mRNA and protein expressions of TfCRT were considerably higher in the gill and liver compared to that of the control treatment. Furthermore, there were no significant differences between Cu-NPs and Cu2+ treatment (at the same treatment concentration) in the expressions of TfCRT at the mRNA or protein levels. Overall, both Cu2+ and Cu-NPs had negative effects on T. fasciatus, and Cu-NPs had the toxic effects similar to dissolved Cu. Our results provide the ecological safety data service of Cu-NPs in water, and provide a reference for the health T. fasciatus aquaculture.