Stable Partial Nitrification At Low Temperature Via Selective Inactivation Of Enzymes By Intermittent Thermal Treatment Of Thickened Sludge

Achieving stable nitrite accumulation at low temperature continues to be a challenging problem in partial nitrification (PN). This study proposes a strategy to achieve stable PN of thickened sludge at low temperatures through selective inactivation of enzymes by intermittent thermal treatment. This method was verified using a sequencing batch reactor (SBR) operated at 9 +/- 1 degrees C in full nitrification mode. After thermally treating the activated sludge at 35 degrees C for 2 days, which was the optimal condition for selective inactivation of enzymes determined by batch tests, an average nitrite accumulation rate (NAR) of 90.18 +/- 5.74% was rapidly achieved and the SBR was stably operated at 9 +/- 1 degrees C for 80 days. This was consistent with a significant decrease of the specific nitrite uptake rate (SNUR), but no a reduction, or even an increase, of the specific ammonia uptake rate (SAUR). Also, there was no remarkable change in the nitrifying bacterial community except for a slight increase in both ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB). In addition, after thermal treatment at 35 degrees C for 2 days, the nitrite oxidoreductase (NOR) activity was significantly decreased, while those of ammonia monooxygenase (AMO) and hydroxylamine oxidoreductase (HAO) showed an increasing trend. Thus, the achievement of stable PN at low temperatures with this strategy may be due to the selective inactivation of NOR, rather than NOB washout.