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Assessment of pollen viability and in vitro pollen germination in Nerium cultivars (Nerium oleander L.)

Parashuram M, Pavan Kumar P,Raghavendra Gunnaiah,Shirol AM, Bapuraygouda P Patil,Md. Jameel Jhalegar,Shankar Meti

International Journal of Chemical Studies(2021)

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Abstract
Pollen viability and in vitro pollen germination was examined in single and double types of Nerium cultivars. Maximum pollen viability per cent was recorded in the cultivar UHSBN-6 (97.90%) followed by the cultivar UHSBN-14 (95.68%) and least per cent was recorded in the cultivar UHSBN-24 (89.58) when methyl blue was used as a dying agent. Potassium iodide showed the maximum per cent pollen viability in the cultivar UHSBN-12 (95.66%). Further, maximum in vitro pollen germination was recorded in the cultivar UHSBN-19 (68.69%) in 15% sucrose with 100 ppm boric acid which was on par with the cultivar UHSBN-17 and UHSBN-29 (67.18 and 66.90% respectively). The cultivar UHSBN-12 recorded maximum in vitro pollen germination (77.12%) under Brew baker and Kwack medium which was on par with cultivars UHSBN-19 and UHSBN-30 (74.80 and 74.72% respectively). The results obtained from the present investigation can aid in planning crosses and establishment of Nerium hybridization programs using compatible cultivars.
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Key words
pollen germination,nerium cultivars,pollen viability
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