Biological control on Tomato Mosaic Virus (ToMV) by using some plant extracts

This study was conducted in Department of Biology, College of Science, University of Anbar, to evaluate the efficiency of plant extracts; Mirabilis jalapa, Eucalyptus, Camellia sinensis to inhibit of tomato mosaic virus proliferation and use these extracts to stimulate the tomato plants resistance against the Tomato Mosaic virus when tested on indicator plants (Datura stramonium) under greenhouse conditions (Saran net shadow). Two concentrations used of each extract crude 100% and 50% concentration, and three replicates used for each treatment and concentration. The results showed that the 50% concentration of both extracts was more efficient to inhibition the proliferation of the Tomato Mosaic virus in 1, 3 and 4 treatments. The results also showed that the Mirabilis jalapa extract was the most efficient to inhibition the virus in all treatments which gave 1.633 (77.660 %) and 2.380 (65.883%) lesion/cm2 in concentration 50 % and crude respectively in the treatment 1 and gave 1.966 (67.786%), 1.136 (82.576%) lesion/cm2 in concentration 50% and crude respectively in the treatment 2 and gave 1.886 ( 74.057%), 2.470 ( 69.201%) lesion/cm2 in concentration 50% and crude respectively in the treatment 3 while the treatment 4 gave number of lesions 1.970 ( 74.827%), 2.353 ( 70.177%) lesion/ cm2 in concentration 50% and crude respectively compared with the distilled water ( control treatment) and with the other plant extracts in our study. Eucalyptus extract was the second level in its efficiency to inhibition of tomato mosaic virus by reduction the number of lesions per square centimeter of the leaf followed by the extract of Camelia sinensis which gave the third level in its ability to inhibit the virus.