Increased Mhc Class I Expression On Platelets In Human And Murine Sepsis

Complications from sepsis are largely driven by maladaptive immune responses. Platelets impact innate and adaptive immune responses, but whether platelets regulate adaptive immune responses in sepsis, including CD8+ T cell responses, remains unknown. To explore a possible contribution of platelets to the altered immune response in sepsis, we used deep sequencing of ribosome-protected RNA (RPR) fragments (also called ribosome footprint profiling) to acquire a global picture of translation in platelets from healthy and septic individuals. Using this method, we identified altered translation of transcripts related to adaptive CD8+T cell responses. We further confirmed these changes using megakaryocyte cultures, and an in vivo model of murine sepsis. Finally, we employed in vitro models to examine their functional relevance.