Mechanical alloying and characterization of chlorine doped hydroxyapatite nanopowders

Chlorine-doped Hydroxyapatite (ClHA)(Ca 10 (PO 4 ) 6 (OH) 2-x Cl x ) nanopowders were successfully synthesized using high planetary ball mill. Milling was carried from 1 to 8 h in order to study the effect of milling time on the doped powders. Additionally, a study on the effect of chlorine addition to HA was also done. XRD results confirmed that all the milled powders consisted apatite as main phase. A significant increase in the unit cell parameters of ClHA nanopowders was observed in contrast to pure HA. However, in doped powders the size decreased with an increase in milling time with a minimal change in the lattice parameters. Microscopic observations also revealed an overall increase in the particle size due to Cl − addition. FTIR results confirmed the incorporation of Cl ions into HA lattice. Hemocompatibility of the synthesized powders (pure HA and ClHA8) was investigated using bovine blood. It was seen that hemolysis ratios for both the samples were below 2%, indicating that the synthesized powders of HA and ClHA are hemocompatible and can be used in various biomedical applications.