Trans Fatty Acid Intake Modulates The Expression Of Uncoupling Proteins 2 And 3 (Ucp2 & Ucp3) Mrna In Children
CURRENT RESEARCH IN NUTRITION AND FOOD SCIENCE(2021)
Abstract
Increased consumption of Trans Fats is associated with increased risk of Coronary Heart Disease. Uncoupling proteins (UCPs) are mitochondrial proteins that disperse the inter-membrane electrochemical potential as heat. We aimed todetect the relation of high Trans-fat intake in diet on the expression of UCP 2 & 3 m-RNA in children. A specific questionnaire to parents of sixty-eight children (4-15 years) was conducted. Accordingly, the subjects were sub-grouped into; High Trans- fat consuming group (37 subjects) and a Medium Trans-fat consuming one (31 subjects). Samples collected from Peripheral blood to analyze UCP 2 & 3 mRNA expression by Real Time Polymerase Chain Reaction (RT-PCR). Levels of UCP2 expression was reduced in children consuming High Trans-fat (2.5 +/- 0.7) in comparison with Medium Trans-fat consuming ones (1.5 +/- 0.2) with (p<0.001). However, not much significance was showed in UCP3 expression with values (2.1 +/- 0.5) in the High consuming group and (1.9 +/- 0.2) in Medium consuming group with (p=0.08). In Delta relationship the diet-induced changes in UCP2 (r= 0.66, P= 0.002) and UCP3 (r=0.61, P=0.06) mRNA expression was negatively correlated with percentage of Trans-fat in diet. The correlation of UCP 2 & 3 mRNA expression and high Trans-fat intake suggests a mechanism by which high Trans-fat diet plays a role in childhood obesity.
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Key words
mRNA Expression, Mitochondrial Uncoupling, Questionnaire, Real Time Polymerase Chain Reaction (RTPCR), Trans Fatty Acids, Uncoupling Proteins (UCPs)
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