Assessment of the Diagnostic Performance of Normal and Irradiated Schistosoma mansoni Cercarial Antigens using ELISA

Irradiation of Schistosoma mansoni cercariae induces structural and motor changes in its surface molecules converting it from poor immunogenic to highly immunogenic surface. This study aims to comparatively analyze normal and gamma radiation-attenuated Schistosoma mansoni cercarial antigens (SCA) by ELISA for serodiagnosis. Total IgG ELISA was assessed to evaluate the potency of two parts of Schistosoma mansoni ceracrial antigens (2000 each) one normal and other exposed to attenuating dose of 400 Gy gamma radiations. The study was conducted on fifty two individual sera samples divided as follows: Group CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I included 21 obtained from patients who were positive for schistosomiasis and negative for other parasitic infections; Group CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN ICYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I included 18 sera from patients who were negative for schistosomiasis and positive for other parasitic infections; (3) fascioliasis, (5) amoebiasis, (7) toxoplasmosis and (3) hydatidosis and Group CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN ICYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN ICYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I included (13) sera from healthy individuals all were diagnosed parasitologically and serologically. Although both antigens achieved 100 % sensitivity and negative predictive value (NPP), irradiated SCA revealed better specificity, accuracy and positive predictive value (PPV) of 87 %, 92.3% and 84 %, respectively. While normal SCA revealed 83.9%, 90.4 % and 80.7 %, respectively. It was concluded that irradiated SCA achieved better remarkable ODs than normal SCA and better specificity due to low cross-reactivity with other parasites. S0 it can be used as a tool for diagnosis of chronic schistosomiasis.