Separation of ctDNA by superparamagnetic bead particles in microfluidic platform for early cancer detection

•CtDNA extraction from blood samples by superparamagnetic (SPM) bead particles in a microfluidic platform for early cancer detection was proposed.•Computer experiments were conducted in COMSOL Multiphysics 5.3a software by using blood samples from stage I and II cancer patients to extract ctDNA.•An average of 5.7 ng of ctDNA was separated for every 10 µL of whole plasma input.•The simulation also showed that the device has ctDNA detection sensitivity and specificity of 65.57% and 95.38%, respectively for samples from stage I and II cancer patients.•The microchannel design with filtration method and separation by SPM bead particles has proved the effective ctDNA extraction from samples of stage I and II cancer patients.