Efficacy of a Biorevitalizing-Filler Solution on All Skin Aspects: 10 Years Approach through <i>in Vitro</i> Studies and Clinical Trials

Introduction: Skin aging is the result of many cellular dysfunctions over time\r\nparticularly the fibroblasts and the keratinocytes. These dysfunctions could be\r\ndecelerated by the preventive effects of some skin treatments such as Intradermal\r\ninjections. NCTF 135HA has a polycomponent formulation designed to improve the efficacy of\r\nnon-cross linked hyaluronic acid (as a micro-filler) on fibroblasts function.\r\nAlthough NCTF 135HA has been used by aesthetic practitioners since 20 years, we\r\nhave analyzed all in vitro, ex vivo and in vivo studies during past 10 years in order to summarized its anti-aging effect. Methods: In these series of studies, the known effects of HA have been evaluated with this\r\nboosted formula. Collagen I synthesis, antiglycation effect and contractile\r\nforces developed by fibroblasts were studied. Fibroblasts and keratinocytes\r\nproliferations were evaluated in monolayer cells culture. The filling effect\r\nthanks to NCTF 135HA injection was ex\r\nvivo performed by fringe projection and the protective effect of NCTF 135HA\r\nagainst solar irradiation was performed ex\r\nvivo to study proliferation rate, elastin and collagen expression. Two\r\nclinical trials were performed on women to evaluate the anti-aging effect of\r\nNCTF 135HA injection. Hydration, firmness, radiance, wrinkles size, pore size,\r\nthickness and density of the dermis were analyzed. Results: Collagen I, anti-AGE products, cell proliferation and contractile forces\r\nwere significantly increased with NCTF 135HA in culture medium. Ex vivo studies showed that NCTF 135HA\r\nincreased, cells proliferation rate, elastin and collagen production. In\r\naddition, the filling effect of the NCTF 135HA is significantly superior to\r\nplacebo. The first clinical trial shows that NCTF 135HA injection significantly\r\nincreased tonicity, hydration and radiance of the skin and significantly\r\ndecreased wrinkle score. The second clinical trial demonstrated that NCTF 135HA\r\ninjection significantly increased dermis density and thickness, skin\r\nhomogeneity and skin radiance and significantly decreased pore size and wrinkle\r\ndepth and volume. Conclusion: In vitro, ex vivo and in vivo studies showed that this minimally invasive technique could be used in order to\r\nimprove the skin quality which could have a visual effect on preventing the\r\naging signs.