Bio-Orthogonal Chemistry Enables Solid Phase Synthesis And Hplc And Gel-Free Purification Of Long Rna Oligonucleotides Dagger

Solid phase synthesis of RNA oligonucleotides which are over 100-nt in length remains challenging due to the complexity of purification of the target strand from failure sequences. This work describes a non-chromatographic strategy that will enable routine solid phase synthesis of long RNA strands.