Stachybotranes A-D, phenylspirodrimanes from the wetland fungus Stachybotrys chartarum with cytotoxic activities

Four new phenylspirodrimanes, stachybotranes A-D (1-4), along with seven known analogues (5-11), were isolated from the extract of a wetland fungal strain Stachybotrys chartarum DTH12-9. The structures of new compounds were determined by spectroscopic analyses, X-ray crystallographic analysis, and the CD analysis of the in situ formed [Rh-2(OCOCF3)(4)] complex. Compounds 1-3, 5-8, and 10 were evaluated for in vitro cytotoxicity against five human cancer cell lines, HL-60, SMMC-7721, A-549, MCF-7, and SW-480. Compounds 1, 2, and 7 exhibited moderate cytotoxic potency against various human cancer cell lines.