Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications.
STAR protocols(2021)
摘要
This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018).
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关键词
Cell Biology,Cell isolation,Flow Cytometry/Mass Cytometry,Immunology,Metabolism
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