Effect of biochar-immobilized Sphingomonas sp. PJ2 on bioremediation of PAHs and bacterial community composition in saline soil
CHEMOSPHERE(2021)
Abstract
This study aimed to investigate the bioremediation efficiency and bacterial regulation mechanism of biochar-immobilized bacterium (BM) in polycyclic aromatic hydrocarbon (PAH)-contaminated saline soil by conducting pot experiments. In BM treatment, PAH-degrading strain Sphingomonas sp. PJ2 was inoculated into biochar produced at 400 degrees C and 600 degrees C using the pine needles (BM400 and BM600). The removal rates of PAHs, soil physicochemical properties, abundance of PAH-ring hydroxylating dioxygenase (PAH-RHD), and bacterial community composition were determined. After 60 days of bioremediation, BM treatment significantly (P < 0.05) increased the removal rate of PAHs compared with biochar and PJ2 alone (15.94% and 37.3%, respectively). BM treatment clearly improved the physicochemical properties of saline soil. Moreover, the amount of Gram-positive PAH degraders increased in BM-treated soils compared with other treatments, and their gene abundance had a strong positive correlation with the removal rates of PAHs in soils (r = 0.896; P < 0.01). Furthermore, BM treatment increased the abundance of Sphingomonas genus, indicating that the strain PJ2 could survive and colonize in PAH-contaminated saline soil under the protection of biochar. This study provided an effective and green approach for the remediation and improvement of the PAH-contaminated saline soil. (C) 2021 Elsevier Ltd. All rights reserved.
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Key words
Biochar-immobilized bacteria,Bioremediation,Ecosystem services,PAHs,Saline soil
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