Anthelmintic activity of a nanoformulation based on thiophenes identified in Tagetes patula L. (Asteraceae) against the small ruminant nematode Haemonchus contortus

•The thiophene isolated from Tagetes patula showed exceptional in vitro anthelmintic activity against multidrug resistant Haemonchus contortus.•The nanoencapsulated compound kept the parasitic load in the digestive tract of the hosts stable, with eggs per gram of feces (EPG) counts having a mean value < 3,000.•Based on the affinity score and the proposed binding mode, the selected thiophene could be classified as a potential GluCl ligand, justifying its exceptional in vitro anthelmintic activity.