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Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-Binding Protein-DNA Complexes

SINGLE STRANDED DNA BINDING PROTEINS(2021)

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Abstract
Optical tweezers enable the isolation and mechanical manipulation of individual nucleoprotein complexes. Here, we describe how to use this technique to interrogate the mechanical properties of individual protein-DNA complexes and extract information about their overall structural organization.
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Key words
Optical tweezers,Single-molecule manipulation,SSB,Single-stranded DNA,Protein-DNA organization
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