Primer, kit and method for detecting low grain protein content of wheat, and applications of method

The invention discloses a primer, a kit and a method for detecting the low grain protein content of wheat, and applications of the method. An upstream primer sequence of the primer is shown as SEQ ID NO: I, and a downstream primer sequence of the primer is shown as SEQ ID NO: II. The method for detecting the low GPC (grain protein content) of wheat provided by the invention is reliable, simple and convenient, and practical, and has a significant application prospect in the wheat genetic resource assessment and the breeding molecular marker-assisted selection, and meanwhile, a reference basis is provided for identifying the wheat variety with the low grain protein content. According to the primer, the kit and the method, the low grain protein content can be screened at the second breeding and hybridization generation, therefore, the defect that in the conventional methods, the low grain protein content only can be screened at the fourth hybridization generation is overcome, thus the working amount of breeders for selecting the characters is reduced, the selecting accuracy and breeding efficiency are improved, and the technology belongs to the novel breeding technology which is efficient and rapid.